Reporter
mRFP1 C-fu
Part:BBa_K537005:Design
Designed by: Gloria Hlongwane Group: iGEM11_WITS-CSIR_SA (2011-09-09)
mCherry (mRFP1) C-Fusion
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 552
Illegal AgeI site found at 664 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
This BioBrick represents a C-terminal fusion part. The part contains a standard suffix and a “assembly standard 25” prefix (Freiburg Fusions/ RFC 25). This version of the mRFP reporter (BBa_E1010) has the standard BioBrick suffix and prefix. The standard Biobrick prefix, however, is followed by another restriction site – specific for NgoMIV (which forms the Freiburg fusion prefix). The part lacks an ATG start codon (provided by N-terminal part). It can therefore be fused to any gene in a modular fashion via the modular construction strategy of protein fusion developed by the Freiburg 2007 team.
Source
The mRFP1 fluorescent reporter is derived from part BBa_E1010